An Olio of Omics: Nature’s Chemical Compositional Complexity Resolved and Identified by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
Alan Marshall, Ph.D.
Most mass analysis relies on “nominal” mass accuracy (i.e., to within 1 Da). However, more and more applications are based on much more accurate mass measurement. Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) offers 10-1000 times higher mass resolving power than other mass analyzers.
High mass resolving power (m/Dm50% > 1,000,000) offers two major advantages: (a) it becomes possible to separate complex mixtures without prior chromatographic or gel separation; and (b) elemental composition may be determined from accurate (to within less than 100 ppb) mass measurement alone for unknown molecules up to ~1,000 Da. Examples from environmental, petrochemical, analytical, and biological problems will be presented. Work supported by NSF Cooperative Agreement No. DMR-1644779 and the State of Florida.